Knockdown of adipokinetic hormone synthesis increases susceptibility to oxidative stress in Drosophila – A role for dFoxO?

0444024 - BC 2016 RIV US eng J - Journal Article
Bednářová, Andrea - Kodrík, Dalibor - Krishnan, N.
Knockdown of adipokinetic hormone synthesis increases susceptibility to oxidative stress in Drosophila – A role for dFoxO?
Comparative Biochemistry and Physiology Part C. Roč. 171, May 01 (2015), s. 8-14. ISSN 1532-0456. E-ISSN 1878-1659
R&D Projects: GA ČR GA14-07172S; GA MŠMT(CZ) LH14047
Grant - others:GA JU(CZ) 140/2014/P
Institutional support: RVO:60077344
Keywords : adipokinetic hormone * Drosophila * hydrogen peroxide
Subject RIV: ED - Physiology
Impact factor: 2.546, year: 2015
http://www.sciencedirect.com/science/article/pii/S1532045615000265

Signaling pathways of insect adipokinetic hormones (AKHs) in anti-oxidative response are unclear. Exposure to hydrogen peroxide (HP) revealed that AKH-RNAi flies showed significantly higher mortality than AKH-oex or control lines. AKH-RNAi flies had significantly less dFoxO transcript and translated protein compared to control and AKH-oex flies in un-challenged condition as well as when challenged with HP. Sestrin – a major antioxidant defense protein and one of the targets of dFoxO – was also significantly down-regulated in AKH-RNAi flies (both unchallenged and challenged with HP) compared to control flies and flies with over-expressed AKH. These findings imply that dFoxO may act downstream of AKH as a transcription factor to mediate response to OS in D. melanogaster.
Permanent Link: http://hdl.handle.net/11104/0248629