Immunohistochemical Analysis of Collagen IV and Laminin Expression in Spontaneous Melanoma Regression in the Melanoma-Bearing Libechov Minipig

0443826 - ÚŽFG 2016 RIV DE eng J - Journal Article
Plánská, Daniela - Burocziová, Monika - Strnádel, Ján - Horák, Vratislav
Immunohistochemical Analysis of Collagen IV and Laminin Expression in Spontaneous Melanoma Regression in the Melanoma-Bearing Libechov Minipig.
Acta histochemica et cytochemica. Roč. 48, č. 1 (2015), s. 15-26. ISSN 0044-5991. E-ISSN 1347-5800
R&D Projects: GA MŠMT ED2.1.00/03.0124
Institutional support: RVO:67985904 ; RVO:61388971
Keywords : collagen IV * laminin * MeLiM * porcine melanoma * spontaneous regression
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 0.912, year: 2015

Spontaneous regression (SR) of human melanoma is a rare, well-documented phenomenon that is not still fully understood. Its detailed study cannot be performed in patients due to ethical reasons. Using the Melanoma-bearing Libechov Minipig (MeLiM) animals of various ages (from 3 weeks to 8 months) we implemented a long-term monitoring of melanoma growth and SR. We focused on immunohistochemical detection of two important extracellular matrix proteins, collagen IV and laminin, which are associated with cancer. We showed that SR of melanoma is a highly dynamic process. The expression of collagen IV and laminin correlated with changes in population of melanoma cells. Tumours of 3-week-old animals consisted primarily of melanoma cells with a granular expression of collagen IV and laminin around them. Thereafter, melanoma cells were gradually destroyed and tumour tissue was rebuilt into the connective tissue. Collagen IV expression slightly increased in tumours of 10-week-old pigs showing extracellular fibrous appearance. In tumours of older animals, areas lacking melanoma cells demonstrated a low expression and areas still containing melanoma cells a high expression of both proteins. We considered the age of 10 weeks as a turning point in the transition between tumour growth and SR of the MeLiM melanoma.
Permanent Link: http://hdl.handle.net/11104/0246506