DNA and RNA Quadruplex-Binding Proteins

0442425 - BFÚ 2015 CH eng J - Journal Article
Brázda, Václav - Haroniková, Lucia - Liao, J.C.C. - Fojta, Miroslav
DNA and RNA Quadruplex-Binding Proteins.
International Journal of Molecular Sciences. Roč. 15, č. 10 (2014), s. 17493-17517. E-ISSN 1422-0067
R&D Projects: GA ČR(CZ) GBP206/12/G151
Institutional support: RVO:68081707
Keywords : DNA quadruplex * RNA quadruplex * telomere
Subject RIV: BO - Biophysics
Impact factor: 2.862, year: 2014

Four-stranded DNA structures were structurally characterized in vitro by NMR, X-ray and Circular Dichroism spectroscopy in detail. Among the different types of quadruplexes (i-Motifs, minor groove quadruplexes, G-quadruplexes, etc.), the best described are G-quadruplexes which are featured by Hoogsteen base-paring. Sequences with the potential to form quadruplexes are widely present in genome of all organisms. They are found often in repetitive sequences such as telomeric ones, and also in promoter regions and 5' non-coding sequences. Recently, many proteins with binding affinity to G-quadruplexes have been identified. One of the initially portrayed G-rich regions, the human telomeric sequence (TTAGGG)(n), is recognized by many proteins which can modulate telomerase activity. Sequences with the potential to form G-quadruplexes are often located in promoter regions of various oncogenes. The NHE III1 region of the c-MYC promoter has been shown to interact with nucleolin protein as well as other G-quadruplex-binding proteins. A number of G-rich sequences are also present in promoter region of estrogen receptor alpha.
Permanent Link: http://hdl.handle.net/11104/0245240