Colloidally stable surface-modified iron oxide nanoparticles: preparation, characterization and anti-tumor activity

0442140 - ÚMCH 2015 RIV NL eng J - Journal Article
Macková, Hana - Horák, Daniel - Donchenko, G. V. - Andriyaka, V. I. - Palyvoda, O. M. - Chernishov, V. I. - Chekhun, V. F. - Todor, I. N. - Kuzmenko, O. I.
Colloidally stable surface-modified iron oxide nanoparticles: preparation, characterization and anti-tumor activity.
Journal of Magnetism and Magnetic Materials. Roč. 380, 15 April (2015), s. 125-131. ISSN 0304-8853. E-ISSN 1873-4766.
[International Conference on the Scientific and Clinical Applications of Magnetic Carriers /10./. Dresden, 10.06.2014-14.06.2014]
R&D Projects: GA MŠMT 7E12054; GA MŠMT(CZ) LH14318; GA MŠMT(CZ) ED1.1.00/02.0109
EU Projects: European Commission(XE) 259796 - DIATOOLS
Institutional support: RVO:61389013
Keywords : iron oxide nanoparticle * poly(N,N-dimethylacrylamide-co-acrylic acid) * protein oxidation
Subject RIV: CD - Macromolecular Chemistry
Impact factor: 2.357, year: 2015

Maghemite (γ-Fe2O3) nanoparticles were obtained by co-precipitation of Fe(II) and Fe(III) chlorides and subsequent oxidation with sodium hypochlorite and coated with poly(N,N-dimethylacrylamide-co-acrylic acid) [P(DMAAm-AA)]. They were characterized by a range of methods including transmission electron microscopy (TEM), elemental analysis, dynamic light scattering (DLS) and zeta potential measurements. The effect of superparamagnetic P(DMAAm-AA)-γ-Fe2O3 nanoparticles on oxidation of blood lipids, glutathione and proteins in blood serum was detected using 2-thiobarbituric acid and the ThioGlo fluorophore. Finally, mice received magnetic nanoparticles administered per os and the antitumor activity of the particles was tested on Lewis lung carcinoma (LLC) in male mice line С57ВL/6 as an experimental in vivo metastatic tumor model; the tumor size was measured and the number of metastases in lungs was determined. Surface-modified γ-Fe2O3 nanoparticles showed higher antitumor and antimetastatic activities than commercial CuFe2O4 particles and the conventional antitumor agent cisplatin.
Permanent Link: http://hdl.handle.net/11104/0245015