Galactose Oxidase from Fusarium oxysporum - Expression in E. coli and P. pastoris and Biochemical Characterization

0440666 - MBÚ 2015 RIV US eng J - Journal Article
Paukner, R. - Staudigl, P. - Choosri, W. - Sygmund, Ch. - Halada, Petr - Haltrich, D. - Leitner, CH.
Galactose Oxidase from Fusarium oxysporum - Expression in E. coli and P. pastoris and Biochemical Characterization.
PLoS ONE. Roč. 9, č. 6 (2014). ISSN 1932-6203. E-ISSN 1932-6203
Grant - others:Austrian Science Foundation(AT) L 504-B11
Institutional support: RVO:61388971
Keywords : galactose oxidase * gene * Fusarium * gene expression
Subject RIV: CE - Biochemistry
Impact factor: 3.234, year: 2014

A gene coding for galactose 6-oxidase from Fusarium oxysporum G12 was cloned together with its native preprosequence and a C-terminal His-tag, and successfully expressed both in Escherichia coli and Pichia pastoris. The enzyme was subsequently purified and characterized. Among all tested substrates, the highest catalytic efficiency (k(cat)/K-m) was found with 1-methyl-beta-D-galactopyranoside (2.2 mM(-1) s(-1)). The Michaelis constant (K-m) for D-galactose was determined to be 47 mM. Optimal pH and temperature for the enzyme activity were 7.0 and 40 degrees C, respectively, and the enzyme was thermoinactivated at temperatures above 50 degrees C. GalOx contains a unique metalloradical complex consisting of a copper atom and a tyrosine residue covalently attached to the sulphur of a cysteine. The correct formation of this thioether bond during the heterologous expression in E. coli and P. pastoris could be unequivocally confirmed by MALDI mass spectrometry, which offers a convenient alternative to prove this Tyr-Cys crosslink, which is essential for the catalytic activity of GalOx.
Permanent Link: http://hdl.handle.net/11104/0243772