Bilateral elevation of interleukin-6 protein and mRNA in both lumbar and cervical dorsal root ganglia following unilateral chronic compression injury of the sciatic nerve

0440580 - BFÚ 2015 RIV GB eng J - Journal Article
Dubový, P. - Brázda, Václav - Klusáková, I. - Hradilová-Svíženská, I.
Bilateral elevation of interleukin-6 protein and mRNA in both lumbar and cervical dorsal root ganglia following unilateral chronic compression injury of the sciatic nerve.
Journal of Neuroinflammation. Roč. 10, č. 55 (2013). E-ISSN 1742-2094
R&D Projects: GA MŠMT(CZ) ED1.1.00/02.0068
Institutional support: RVO:68081707
Keywords : Unilateral nerve injury * Contralateral reaction * Remote ganglia
Subject RIV: BO - Biophysics
Impact factor: 4.902, year: 2013

Background: Current research implicates interleukin (IL)-6 as a key component of the nervous-system response to injury with various effects. Methods: We used unilateral chronic constriction injury (CCI) of rat sciatic nerve as a model for neuropathic pain. Immunofluorescence, ELISA, western blotting and in situ hybridization were used to investigate bilateral changes in IL-6 protein and mRNA in both lumbar (L4-L5) and cervical (C7-C8) dorsal root ganglia (DRG) following CCI. The operated (CCI) and sham-operated (sham) rats were assessed after 1, 3, 7, and 14 days. Withdrawal thresholds for mechanical hyperalgesia and latencies for thermal hyperalgesia were measured in both ipsilateral and contralateral hind and fore paws.Results: The ipsilateral hind paws of all CCI rats displayed a decreased threshold of mechanical hyperalgesia and withdrawal latency of thermal hyperalgesia, while the contralateral hind and fore paws of both sides exhibited no significant changes in mechanical or thermal sensitivity. No significant behavioral changes were found in the hind and fore paws on either side of the sham rats, except for thermal hypersensitivity, which was present bilaterally at 3 days.
Permanent Link: http://hdl.handle.net/11104/0243708