Tetracycline-inducible gene expression system in Leishmania mexicana

0439435 - BC 2015 RIV NL eng J - Journal Article
Kraeva, N. - Ishemgulova, A. - Lukeš, Julius - Yurchenko, Vyacheslav
Tetracycline-inducible gene expression system in Leishmania mexicana.
Molecular and Biochemical Parasitology. Roč. 198, č. 1 (2014), s. 11-13. ISSN 0166-6851. E-ISSN 1872-9428
R&D Projects: GA MŠMT(CZ) EE2.3.30.0032
Institutional support: RVO:60077344
Keywords : Leishmania mexicana * Gene expression * Tet-inducible system
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 1.787, year: 2014

Here we present a T7-driven, tetracycline-inducible system for protein expression in human pathogen Leishmania mexicana. The gene expression in this strain is tightly regulated and dose- and timedependent. This system can be widely used by the parasitology community to analyze effects of genes of interest on biology, physiology and virulence of parasites causing cutaneous leishmaniases.
Permanent Link: http://hdl.handle.net/11104/0242742