Enzymatic Sensor of Biogenic Amines with Optical Oxygen Transducer

0439186 - ÚCHP 2015 CZ eng C - Conference Paper (international conference)
Maixnerová, Lucie - Horvitz, Alexandar - Kuncová, Gabriela - Přibyl, M. - Šebela, M. - Koštejn, Martin
Enzymatic Sensor of Biogenic Amines with Optical Oxygen Transducer.
Book of Abstracts. Brno: Masarykova Universita, 2014, s. 173. ISBN 978-80-210-7159-9.
[CEITEC Annual Conference. Brno (CZ), 21.10.2014-24.10.2014]
R&D Projects: GA TA ČR TA03010544
Institutional support: RVO:67985858
Keywords : optical enzymatic biosensor * biogenic amines * mathematical model
Subject RIV: CB - Analytical Chemistry, Separation

The biogenic amines (BA) are formed by the decarboxylation of amino acids. Because of their impact on human health, they have been used as markers for assessing the freshness and quality of a wide variety of protein-containing products. We studied biosensor for BA with an optical oxygen transducer that contained a diamine oxidase from pea and ruthenium complex. Both active components were incorporated in chemically stable polymer to form sensitive films thickness of 100 - 200 μm. With aim to optimize a composition and manufacturing of the biosensor we developer a mathematical model of processes inside the sensing film. The developed mathematical model revealed qualitative dependencies of the biosensor behavior (sensitivity, response time and dynamic range) on the (i) thickness of the sensitive layer, (ii) concentrations of substrates in the solution, (iii) enzyme activity in the layer and (iv) layer permeability for the substrates.
Permanent Link: http://hdl.handle.net/11104/0242475