Separation of nucleobases, nucleosides, and nucleotides using two zwitterionic silica-based monolithic capillary columns coupled with tandem mass spectrometry

0438643 - ÚIACH 2015 RIV NL eng J - Journal Article
Moravcová, Dana - Haapala, M. - Planeta, Josef - Hyötyläinen, T. - Kostiainen, R. - Wiedmer, S. K.
Separation of nucleobases, nucleosides, and nucleotides using two zwitterionic silica-based monolithic capillary columns coupled with tandem mass spectrometry.
Journal of Chromatography A. Roč. 1373, Dec (2014), s. 90-96. ISSN 0021-9673. E-ISSN 1873-3778
R&D Projects: GA MV VG20112015021; GA ČR(CZ) GAP106/12/0522
Institutional support: RVO:68081715
Keywords : HILIC separation * ESI–MS * Monolithic silica column
Subject RIV: CB - Analytical Chemistry, Separation
Impact factor: 4.169, year: 2014

tThe capability of employing synthesized zwitterionic silica-based monolithic capillary columns(140 mm × 0.1 mm) for separation of highly polar and hydrophilic nucleobases, nucleosides, andnucleotides in hydrophilic interaction chromatography is reported. The suitability of the columns foron-line conjunction with electrospray tandem mass spectrometry was explored. Our results showthat the grafted layer of zwitterionic monomer ([2-(methacryloyloxy)ethyl]-dimethyl-(3-sulfopropyl)-ammonium hydroxide or 2-methacryloyloxyethyl phosphorylcholine) on the silica monolithic surfacesignificantly improved the separation selectivity and reproducibility, as compared to the bare silica mono-lith. The stepwise elution from 90% to 70% of acetonitrile enabled separation of a complex sample mixturecontaining 21 compounds with a total analysis time less than 40 min.
Permanent Link: http://hdl.handle.net/11104/0242051