0438211 - ÚOCHB 2015 RIV US eng J - Journal Article
Dziuba, Dmytro - Pohl, Radek - Hocek, Michal
Bodipy-Labeled Nucleoside Triphosphates for Polymerase Synthesis of Fluorescent DNA.
Bioconjugate Chemistry. Roč. 25, č. 11 (2014), s. 1984-1995. ISSN 1043-1802. E-ISSN 1520-4812
R&D Projects: GA ČR GBP206/12/G151
Institutional support: RVO:61388963
Keywords : enzyme amplification reaction * thymidine nucleotide analog * living cells
Subject RIV: CC - Organic Chemistry
Impact factor: 4.513, year: 2014

New fluorescent nucleosides and nucleoside triphosphate (dNTPs) analogs bearing the F-Bodipy fluorophore linked through a short, flexible nonconjugate tether were synthesized. The Bodipy-labeled dNTPs were substrates for several DNA polymerases which incorporated them into DNA in primer extension, nicking enzyme amplification reaction, and polymerase chain reaction. The fluorescence of F-Bodipy is not quenched upon incorporation in DNA and can be detected both in solutions and on gels.
Permanent Link: http://hdl.handle.net/11104/0241676