Sample preparation for single cell analysis

0437989 - ÚIACH 2015 RIV CZ eng C - Conference Paper (international conference)
Adamová, Eva - Basova, E. Y. - Potáčová, Anna - Foret, František - Matalová, Eva - Klepárník, Karel
Sample preparation for single cell analysis.
CECE 2014. 11th International Interdisciplinary Meeting on Bioanalysis. Brno: Institute of Analytical Chemistry AS CR, 2014 - (Foret, F.; Křenková, J.; Drobníková, I.; Guttman, A.; Klepárník, K.), s. 118-121. ISBN 978-80-904959-2-0.
[CECE 2014. International Interdisciplinary Meeting on Bioanalysis /11./. Brno (CZ), 20.10.2014-22.10.2014]
R&D Projects: GA ČR(CZ) GA14-28254S; GA MŠMT(CZ) EE2.3.20.0182; GA ČR(CZ) GBP206/12/G014
Institutional support: RVO:68081715
Keywords : single cell analysis * micromanipulation * bioluminiscence
Subject RIV: CB - Analytical Chemistry, Separation
http://www.ce-ce.org/CECE2014/CECE%202014%20proceedings_full.pdf

Development of sensitive, miniaturized and fast methods aims to reliable analyze even small amounts of samples. Micromanipulation systems such as laser capture microdissection enable to collect just single cells from cell suspensions or tissue samples. One option for further analysis is the bioluminescence reaction based on luciferin/luciferase conversion. This approach was used for detection of active caspases, caspase-3/7. Droplets based microfluidic systems provide benefits in biological, pharmaceutical and chemical research.
Permanent Link: http://hdl.handle.net/11104/0241477