Application of Fӧrster resonance energy transfer (FRET) for a detection of DNA mutations

0437861 - ÚIACH 2015 BR eng A - Abstract
Datinská, Vladimíra - Klepárník, Karel - Belšánová, Barbora - Minárik, M. - Foret, František
Application of Fӧrster resonance energy transfer (FRET) for a detection of DNA mutations.
ITP & LACE 2014. Book of Abstracts. Grupo VLS Print Solution, 2014 - (Guzman, N.; Taveres, M.). s. 93-93
[International Symposium on Electro- and Liquid Phase-Separation Techniques /21./ and Latin-American Symposium on Biotechnology, Biomedical, Biopharmaceutical, and Industrial Applications of Capillary Electrophoresis and Microchip Technology /21./. 04.10.2014-08.10.2014, Natal]
R&D Projects: GA ČR(CZ) GA14-28254S; GA TA ČR(CZ) TA02010672
Institutional support: RVO:68081715
Keywords : quantum dots * Förster resonance energy transfer * CE-LIF
Subject RIV: CB - Analytical Chemistry, Separation

We have synthesized a probe consisting of CdTe QDs conjugated with oligonucleotide to be used as a donor. Conjugation reaction between carboxylated QDs and aminated oligonucleotides has been performed via zero-length cross-linkers. Sample consisting of clinically-relevant PCR amplicons labelled with ROX (6-carboxyrhodamine) served as acceptor. Upon mutation-specific sample-probe hybridization an increase in fluorescence intensity at 610 nm (the emission spectra maximum of ROX) confirmed a proper function of FRET at a presence of detected mutation. The limit of detection evaluated for PCR amplified fragments of the sample is 4x10-9 M.
Permanent Link: http://hdl.handle.net/11104/0241332