Electrophoretic preconcentration and separation of cationic labeled saccharides

0437860 - ÚIACH 2015 BR eng A - Abstract
Partyka, Jan - Foret, František
Electrophoretic preconcentration and separation of cationic labeled saccharides.
ITP & LACE 2014. Book of Abstracts. Grupo VLS Print Solution, 2014 - (Guzman, N.; Taveres, M.). s. 97-97
[International Symposium on Electro- and Liquid Phase-Separation Techniques /21./ and Latin-American Symposium on Biotechnology, Biomedical, Biopharmaceutical, and Industrial Applications of Capillary Electrophoresis and Microchip Technology /21./. 04.10.2014-08.10.2014, Natal]
R&D Projects: GA ČR(CZ) GAP301/11/2055
Institutional support: RVO:68081715
Keywords : saccharide * AETMA * electrophoresis
Subject RIV: CB - Analytical Chemistry, Separation

In continuation of this work we have been exploring small molecules with permanent positive charge as cationic tags. Oligosaccharides and glycans were labeled by reductive amination and the resulting derivatized saccharides were analyzed by capillary electrophoresis coupled to contactless conductivity detection and electrospray ionization mass spectrometry. The quaternary ammonium based tags assured migration of the oligosaccharide species as cations with electrophoretic mobility independent of the pH of the background electrolyte. For transient isotachophoretic preconcentration, the sample was complemented with ammonium ions and injected as a long zone occupying up to 70% of the separation capillary length. The labeled species, injected in the nanomolar concentrations, were focused into narrow zones by transient isotachophoresis followed by the separation in capillary electrophoretic mode.
Permanent Link: http://hdl.handle.net/11104/0241333