0436873 - ÚJF 2015 RIV US eng J - Journal Article
Pachnerová Brabcová, Kateřina - Sihver, L. - Yasuda, N. - Matuo, Y. - Štěpán, Václav - Davídková, Marie
Clustered DNA damage on subcellular level: effect of scavengers.
Radiation and Environmental Biophysics. Roč. 53, č. 4 (2014), s. 705-712. ISSN 0301-634X. E-ISSN 1432-2099
R&D Projects: GA MŠMT LD12008
Institutional support: RVO:61389005
Keywords : clustered damage * indirect effects * haevy ion * plasmid in liquid water * scavenger
Subject RIV: BO - Biophysics
Impact factor: 1.528, year: 2014 ; AIS: 0.528, rok: 2014
DOI: https://doi.org/10.1007/s00411-014-0557-2

Clustered DNA damages are induced by ionizing radiation, particularly of high linear energy transfer (LET). Compared to isolated DNA damage sites, their biological effects can be more severe. We investigated a clustered DNA damage induced by high LET radiation (C 290 MeV u(-1) and Fe 500 MeV u(-1)) in pBR322 plasmid DNA. The plasmid is dissolved in pure water or in aqueous solution of one of the three scavengers (coumarin-3-carboxylic acid, dimethylsulfoxide, and glycylglycine). The yield of double strand breaks (DSB) induced in the DNA plasmid-scavenger system by heavy ion radiation was found to decrease with increasing scavenging capacity due to reaction with hydroxyl radical, linearly with high correlation coefficients. The yield of non-DSB clusters was found to occur twice as much as the DSB. Their decrease with increasing scavenging capacity had lower linear correlation coefficients. This indicates that the yield of non-DSB clusters depends on more factors, which are likely connected to the chemical properties of individual scavengers.
Permanent Link: http://hdl.handle.net/11104/0240517