The X Chromosome Is Necessary for Somatic Development in the Dioecious Silene latifolia: Cytogenetic and Molecular Evidence and Sequencing of a Haploid Genome

0436672 - BFÚ 2015 RIV CH eng J - Journal Article
Soukupová, Magda - Nevrtalová, Eva - Čížková, Jana - Vogel, Ivan - Čegan, Radim - Hobza, Roman - Vyskot, Boris
The X Chromosome Is Necessary for Somatic Development in the Dioecious Silene latifolia: Cytogenetic and Molecular Evidence and Sequencing of a Haploid Genome.
Cytogenetic and Genome Research. Roč. 143, 1-3 (2014), s. 96-103. ISSN 1424-8581. E-ISSN 1424-859X
R&D Projects: GA ČR(CZ) GBP501/12/G090; GA ČR(CZ) GAP501/12/2220; GA ČR(CZ) GAP501/10/0102; GA MŠMT(CZ) LM2010005; GA MŠMT LO1204
Institutional support: RVO:68081707
Keywords : Anther culture * Cytometry * Dihaploids
Subject RIV: BO - Biophysics; EF - Botanics (UEB-Q)
Impact factor: 1.561, year: 2014

Silene latifolia (or white campion) possesses a well-established sex determination system with a dominant Y chromosome in males (the mammalian type). The heteromorphic sex chromosomes X and Y in S. latifolia largely stopped recombination; thus, we can expect a gradual genetic degeneration of the Y chromosome. It is well proven that neither diploid nor polyploid S. latifolia sporophytes can survive without at least one X, so the only life stage possessing the Y as the sole sex chromosome is the male gametophyte (pollen tube), while the female gametophyte seems to be X-dependent. Previous studies on anther-derived plants of this species showed that the obtained plants (largely haploid or dihaploid) were phenotypically and cytologically female. In this paper, we provide molecular evidence for the inviability of plants lacking the X chromosome. Using sex-specific PCR primers, we show that all plantlets and plants derived from anther cultures are female. In studying anther-derived diploid females by sequencing of X-linked markers, we demonstrate that these plants are really homozygous dihaploids. A haploid regenerant plant was sequenced (8x genome coverage) using Illumina technology.
Permanent Link: http://hdl.handle.net/11104/0240393