Mapping of single-copy genes by TSA-FISH in th codling moth, Cydia pomonella

0436606 - BC 2015 RIV GB eng J - Journal Article
Carabajal Paladino, Leonela Z. - Nguyen, Petr - Šíchová, Jindra - Marec, František
Mapping of single-copy genes by TSA-FISH in th codling moth, Cydia pomonella.
B M C Genetics. Roč. 15, Supplement 2 (2014), S15. ISSN 1471-2156. E-ISSN 1471-2156
R&D Projects: GA ČR(CZ) GA14-22765S; GA ČR(CZ) GP14-35819P; GA MŠMT(CZ) EE2.3.30.0032
Grant - others:GA JU(CZ) 052/2013/P; IAEA, Vienna(AT) 15838
Institutional support: RVO:60077344
Keywords : Cydia pomonella
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 2.397, year: 2014
http://www.biomedcentral.com/content/pdf/1471-2156-15-S2-S15.pdf

In this study, we adapted a FISH protocol with tyramide signal amplification (TSA-FISH) for detection of single-copy genes in Lepidoptera. We tested the protocol with probes prepared from partial sequences of Z-linked genes in the codling moth. Using a modified TSA-FISH protocol we successfully mapped a partial sequence of the Acetylcholinesterase 1 (Ace-1) gene to the Z chromosome and confirmed thus its Z-linkage. A subsequent combination of BAC-FISH with BAC probes containing anticipated neighbouring Z-linked genes and TSA-FISH with the Ace-1 probe allowed the integration of Ace-1 in the physical map of the codling moth Z chromosome. We also developed a two-colour TSA-FISH protocol which enabled us simultaneous localization of two Z-linked genes, Ace-1 and Notch, to the expected regions of the Z chromosome. Our results suggest that TSA-FISH represents a reliable technique for physical mapping of genes or localization of transgene cassettes on chromosomes of moths and butterflies.
Permanent Link: http://hdl.handle.net/11104/0240402