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Nonhomologous DNA end joining and chromosome aberrations in human embryonic lung fibroblasts treated with environmental pollutants
- 1.0435644 - ÚEM 2015 RIV NL eng J - Journal Article
Rössner ml., Pavel - Rössnerová, Andrea - Beskid, Olena - Tabashidze, Nana - Líbalová, Helena - Uhlířová, Kateřina - Topinka, Jan - Šrám, Radim
Nonhomologous DNA end joining and chromosome aberrations in human embryonic lung fibroblasts treated with environmental pollutants.
Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis. 763-764, MAY-JUN 2014 (2014), s. 28-38. ISSN 0027-5107
R&D Projects: GA ČR GAP503/11/0084
Institutional support: RVO:68378041
Keywords : benzo[a]pyrene * chromosome aberrations * double-strand DNA breaks
Subject RIV: DN - Health Impact of the Environment Quality
Impact factor: 3.680, year: 2014
The cells were treated for 24 h with B[a]P (1 μM, 10 μM, 25 μM) and EOMs (1 μg/ml, 10 μg/ml, 25 μg/ml). 25 μM of B[a]P and most of the tested doses of EOMs induced double strand breaks (DSBs) as indicated by H2AX phosphorylation. DNA damage was accompanied by induction of XRCC4 expression and an increased frequency of chromosomal aberrations. Translocations most frequently affected chromosome 7. We observed only a weak induction of Ku70/80 expression as well as ligation activity of nuclear extracts.
Permanent Link: http://hdl.handle.net/11104/0239451
Number of the records: 1