Depolarization affects lateral microdomain structure of yeast plasma membrane

0435550 - ÚEM 2016 RIV GB eng J - Journal Article
Herman, P. - Večeř, J. - Opekarová, Miroslava - Veselá, Petra - Jančíková, I. - Zahumenský, J. - Malínský, Jan
Depolarization affects lateral microdomain structure of yeast plasma membrane.
FEBS Journal. Roč. 282, č. 3 (2015), s. 419-434. ISSN 1742-464X. E-ISSN 1742-4658
R&D Projects: GA ČR GAP205/12/0720
Institutional support: RVO:68378041
Keywords : gel microdomains * lipid order * transmembrane potential
Subject RIV: EA - Cell Biology
Impact factor: 4.237, year: 2015

We report the transmembrane voltage-induced lateral reorganization of highly-ordered lipid microdomains in the plasma membrane of living yeast. Different depolarization mechanisms, including the application of ionophores, cell depolarization by an external electric field, and partial depolarization by protein-mediated ion transport or by compromised respiration efficiency, yielded the same results: a significant reduction in the amount of gel-like microdomains in the membrane. The data suggest that the voltage-induced reorganization of lateral membrane structure could play significant role in fast cellular response to acute stress conditions, as well as in other membrane microdomain-related regulatory mechanisms.
Permanent Link: http://hdl.handle.net/11104/0245098