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Droplet microfluidics in (bio) chemical analysis

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    0434461 - ÚIACH 2015 RIV GB eng J - Journal Article
    Basova, E. Y. - Foret, František
    Droplet microfluidics in (bio) chemical analysis.
    Analyst. Roč. 140, č. 1 (2015), s. 22-38. ISSN 0003-2654. E-ISSN 1364-5528
    R&D Projects: GA ČR(CZ) GBP206/12/G014
    Institutional support: RVO:68081715
    Keywords : droplet chemistry * bioanalysis * microfluidics * protein
    Subject RIV: CB - Analytical Chemistry, Separation
    Impact factor: 4.033, year: 2015

    Droplet microfluidics may soon change the paradigm of performing chemical analyses and related instrumentation. It is not only the analysis scale and possibility for sensitivity improvement, reduced consumption of chemical and biological reagents, but also the vast increase of the speed in performing a variety of unit operations. At present, microfluidic platforms can reproducibly generate monodisperse droplet populations at kHz or higher rate with droplet sizes suitable for high-throughput experiments, single-cell detection or even single molecule analysis. In addition to being used as microreactors with volume in the micro- to femtoliter range; droplet based systems have also been used to directly synthesize particles and encapsulate biological entities for biomedicine and biotechnology applications. This minireview summarizes the various droplet microfluidics operations and applications for (bio) chemical assays described in the literature during the past few years.
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    0434461_IR.pdf141.4 MBAuthor’s postprintrequire
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