Peroxidase-like activity of magnetoferritin

0434307 - ÚVGZ 2015 RIV AT eng J - Journal Article
Melníková, V. - Pospíšková, K. - Mitróová, Z. - Kopčanský, P. - Šafařík, Ivo
Peroxidase-like activity of magnetoferritin.
Microchimica Acta. Roč. 181, 3-4 (2014), s. 295-301. ISSN 0026-3672. E-ISSN 1436-5073
R&D Projects: GA MŠMT(CZ) LD13021
Institutional support: RVO:67179843
Keywords : magnetoferritin * magnetic nanoparticles * peroxidase-like activity * hydrogen peroxide * oxidative stress
Subject RIV: CE - Biochemistry
Impact factor: 3.741, year: 2014

Magnetoferritin is a spherical biomacromolecule with a diameter of about 12 nm. It consists of a protein shell composed of apoferritin that is surrounding magnetic nanoparticles of magnetite (Fe3O4) or maghemite (gamma-Fe2O3). Magnetoferritins with various iron content (loading factor) were synthetically prepared and their peroxidase-like activities studied via the oxidation of the chromogenic substrate N,N-diethyl-p-phenylenediamine sulfate by hydrogen peroxide to give a purple product with an absorption maximum at 551 nm. Magnetoferritin with higher loading factor exhibits a higher peroxidase-like activity. The catalytic activity was successfully applied to the determination of hydrogen peroxide in the 5.8 to 88.2 mM concentration range.
Permanent Link: http://hdl.handle.net/11104/0238392