How to Preserve Plant Samples for Carbohydrate Analysis? Test of Suitable Methods Applicable in Remote Areas

0432919 - BÚ 2015 RIV NL eng J - Journal Article
Chlumská, Zuzana - Janeček, Štěpán - Doležal, Jiří
How to Preserve Plant Samples for Carbohydrate Analysis? Test of Suitable Methods Applicable in Remote Areas.
Folia Geobotanica. Roč. 49, č. 1 (2014), s. 1-15. ISSN 1211-9520. E-ISSN 1874-9348
R&D Projects: GA AV ČR IAA600050802; GA ČR GA526/09/0963; GA ČR GD206/08/H044; GA ČR GA526/07/0808
Institutional support: RVO:67985939
Keywords : ethanol extraction * liquid nitrogen * nonstructural carbohydrates
Subject RIV: EF - Botanics
Impact factor: 1.778, year: 2014

Normally the plants tissues subjected to nonstructural carbohydrate analyses are stored in Dewar flasks with liquid nitrogen. This cannot be applied in remote areas, such as high mountains, because flasks are very heavy and liquid nitrogen evaporates in a few weeks. We invented a new method, which is much more suitable for field conditions - plant tissues can only be boiled in hot 50% ethanol, using simple equipment. This fully ensures denaturation of enzymes and plants tissues can be easily transported in small test tubes and can be stored for more than a month.
Permanent Link: http://hdl.handle.net/11104/0237251