Hydrogen-terminated diamond sensors for electrical monitoring of cells

0432182 - FZÚ 2015 RIV CH eng C - Conference Paper (international conference)
Ižák, Tibor - Novotná, Katarína - Kopová, Ivana - Bačáková, Lucie - Varga, Marián - Rezek, Bohuslav - Kromka, Alexander
Hydrogen-terminated diamond sensors for electrical monitoring of cells.
Key Engineering Materials : Materials and Applications for Sensors and Transducers III. Vol. 605. Zurich: Trans Tech Publications, 2014 - (Hristoforou, E.; Vlachos, D.), s. 577-580. ISBN 9783038350514. ISSN 1013-9826.
[International Conference on Materials and Applications for Sensors and Transducers /3./ (IC-MAST 2013). Praha (CZ), 13.09.2013-17.09.2013]
R&D Projects: GA ČR(CZ) GAP108/11/0794
Institutional support: RVO:68378271 ; RVO:67985823
Keywords : diamond thin films * label-free biosensors * real-time monitoring * cell cultivation * impedance measurements
Subject RIV: JB - Sensors, Measurment, Regulation

In this paper we introduce fully optically transparent impedance biosensors based on intrinsic nanocrystalline diamond (NCD) films deposited on glass substrate. Prepared sensors have an interdigital electrode (IDE) structures realized by local hydrogen and oxygen termination of diamond surface, which mean in-plane configuration of active sensor area. Sensors were tested by real time monitoring of human osteoblast-like MG 63 cells in wide frequency range from 10 Hz to 100 kHz for several days. Two different measurement setups were used and compared regarding to their advantages and disadvantages. Proof of concept of diamond-based impedance sensor is showed, i.e. time dependence and frequency dependence (Nyquist plots) of absolute impedance.
Permanent Link: http://hdl.handle.net/11104/0236646