Counting cell number in situ by quantification of dimethyl sulphide in culture headspace

0431395 - ÚFCH JH 2015 RIV GB eng J - Journal Article
Chippendale, T. W. E. - Španěl, Patrik - Smith, D. - El Haj, A.
Counting cell number in situ by quantification of dimethyl sulphide in culture headspace.
Analyst. Roč. 139, JUL 2014 (2014), s. 4903-4907. ISSN 0003-2654. E-ISSN 1364-5528
Institutional support: RVO:61388955
Keywords : TUBE MASS-SPECTROMETRY * SIFT-MS * VOLATILE COMPOUNDS
Subject RIV: CF - Physical ; Theoretical Chemistry
Impact factor: 4.107, year: 2014

A novel, non-invasive technique is reported for determining the numbers of cells in a culture by quantifying dimethyl sulphide (DMS) in the culture headspace as produced by the cellular enzymatic reduction of dissolved dimethyl sulphoxide (DMSO). Measured DMS concentrations, as performed using selected ion flow tube mass spectrometry (SIFT-MS), in the headspace of 2D and 3D cultures of four cell lines, viz. HEK293 (kidney), MG63 (bone), hepG2 (liver) and CALU-1 (lung), linearly correlate with starting cell number. Clear differences in the rates of production of DMS by the four cell types in both the 2D and 3D situations are seen. This novel analytical technique for cell enumeration offers a significant contribution to quality assessment across cell-based research and industry, including analysis of large scale culture systems, and for routine cell biology research.
Permanent Link: http://hdl.handle.net/11104/0235934