Significantly higher occurrence of Cryptosporidium infection in Roma children compared with non-Roma children in Slovakia

0430920 - BC 2015 RIV US eng J - Journal Article
Hasajová, A. - Valenčáková, A. - Malečková, B. - Danišová, D. - Halán, M. - Goldová, M. - Sak, Bohumil - Květoňová, Dana - Kváč, Martin - Halanová, M.
Significantly higher occurrence of Cryptosporidium infection in Roma children compared with non-Roma children in Slovakia.
European Journal of Clinical Microbiology & Infectious Diseases. Roč. 33, č. 8 (2014), s. 1401-1406. ISSN 0934-9723. E-ISSN 1435-4373
Institutional support: RVO:60077344
Keywords : molecular characterization * immunocompetent man * transmission * Murisinfection
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 2.668, year: 2014

Cryptosporidiosis is considered to be a widespread world zoonosis. The occurrence of Cryptosporidium species was investigated in Roma children in a district of Eastern Slovakia and, at the same time, also in children of non-Roma parents. In total, 103 children (54 boys and 49 girls) between 0 and 14 years of age were involved in this study. Fifty-three were Roma children and 50 children represented a non-Roma control group. Fecal samples were examined: immunologically [enzyme-linked immunosorbent assay (ELISA) test to prove antigen in the feces] and by molecular analysis [nested polymerase chain reaction (PCR)]. After the sequencing of the PCR, the products were identified as species of Cryptosporidium muris. Based on the results, the relative risk (RR) of the Cryptosporidium infection occurrence was calculated and we came to the conclusion that the risk of Cryptosporidium infection was almost 12 times higher in the Roma children compared to the non-Roma children.
Permanent Link: http://hdl.handle.net/11104/0235580