Electrochemical analysis of DNA using switchable redox moieties

0429318 - ÚOCHB 2015 CZ eng C - Conference Paper (international conference)
Fojta, Miroslav - Daňhel, Aleš - Horáková Brázdilová, Petra - Plucnara, Medard - Pivoňková, Hana - Havran, Luděk - Vidláková, Pavlína - Raindlová, Veronika - Balintová, Jana - Macíčková-Cahová, Hana - Hocek, Michal
Electrochemical analysis of DNA using switchable redox moieties.
Chemistry of Nucleic Acid Components. 16th Symposium. Praha: Institute of Organic Chemistry and Biochemistry AS CR, v. v. i, 2014 - (Hocek, M.), s. 264-266. Collection Symposium Series, 14. ISBN 978-80-86241-50-0.
[Symposium on Chemistry of Nucleic Acid Components /16./. Český Krumlov (CZ), 08.06.2014-13.06.2014]
R&D Projects: GA ČR GBP206/12/G151
Grant - others:OPVK(CZ) CZ.1.07/2.3.00/30.0019
Institutional support: RVO:61388963 ; RVO:68081707
Keywords : DNA * electrochemical analysis * switchable redox moieties
Subject RIV: CC - Organic Chemistry; BO - Biophysics (BFU-R)

Labelling of DNA with electrochemically active moieties proved to be a convenient way to the development of electrochemical techniques for the sequence-specific DNA sensing. Through combinations of various labels differing in redox potentials, independent redox coding of different DNA sequences or individual nucleobases can be attained. Applications possibilities of electrochemistry in analysis of modified DNAs are further extended by facile monitoring of chemical conversion of reactive groups on DNA during post-labelling with ultimate redox labels. In addition, controlled in situ electrochemical conversions of specific intrinsic and extrinsic DNA components can be utilized to switch their electrochemical signals and improve signal resolution.
Permanent Link: http://hdl.handle.net/11104/0234537