Number of the records: 1
High- and low-affinity sites for sodium in delta-OR-G(i)1 alpha (Cys(351)-Ile(351)) fusion protein stably expressed in HEK293 cells; functional significance and correlation with biophysical state of plasma membrane
- 1.0428435 - FGÚ 2015 RIV DE eng J - Journal Article
Vošahlíková, Miroslava - Jurkiewicz, Piotr - Roubalová, Lenka - Hof, Martin - Svoboda, Petr
High- and low-affinity sites for sodium in delta-OR-G(i)1 alpha (Cys(351)-Ile(351)) fusion protein stably expressed in HEK293 cells; functional significance and correlation with biophysical state of plasma membrane.
Naunyn-Schmiedeberg's Archives of Pharmacology. Roč. 387, č. 5 (2014), s. 487-502. ISSN 0028-1298. E-ISSN 1432-1912
R&D Projects: GA ČR(CZ) GAP207/12/0919; GA ČR(CZ) GBP304/12/G069
Institutional support: RVO:67985823 ; RVO:61388955
Keywords : delta - opioid receptor * monovalent ions * agonist and antagonist binding * [35S]GTPγS binding * membrane biophysics * Laurdan fluorescence
Subject RIV: CE - Biochemistry
Impact factor: 2.471, year: 2014
The effect of monovalent cations on delta-opioid receptor ligand binding parameters and coupling with the cognate G protein was compared in model HEK293 cell line stably expressing PTX-insensitive delta OR Gi1alpha (Cys351-Ile351) fusion protein. The possible differences between individual ions when interacting with the polar membrane-water interface and the hydrophobic interior of membrane bilayer was studied with help of fluorescence probes
Permanent Link: http://hdl.handle.net/11104/0233793
Number of the records: 1