Nuclear Structures Surrounding Internal Lamin Invaginations

0427969 - BFÚ 2015 RIV US eng J - Journal Article
Legartová, Soňa - Stixová, Lenka - Laur, O. - Kozubek, Stanislav - Sehnalová, Petra - Bártová, Eva
Nuclear Structures Surrounding Internal Lamin Invaginations.
Journal of Cellular Biochemistry. Roč. 115, č. 3 (2014), s. 476-487. ISSN 0730-2312. E-ISSN 1097-4644
R&D Projects: GA MŠMT(CZ) LD11020
Institutional support: RVO:68081707
Keywords : LAMINS * NUCLEAR PORES * CHROMATIN
Subject RIV: BO - Biophysics
Impact factor: 3.263, year: 2014

A- and C-type lamins are intermediate filament proteins responsible for the maintenance of nuclear shape and most likely nuclear architecture. Here, we propose that pronounced invaginations of A/C-type lamins into the nuclear interior represent channels for the transport of regulatory molecules to and from nuclear and nucleolar regions. Using fluorescent protein technology and immunofluorescence, we show that A-type lamin channels interact with several nuclear components, including fibrillarin- and UBF-positive regions of nucleoli, foci of heterochromatin protein 1 , polycomb group bodies, and genomic regions associated with DNA repair. Similar associations were observed between A/C-type lamin channels and nuclear pores, lamin-associated protein LAP2, and promyelocytic leukemia nuclear bodies. Interestingly, regions with high levels of A/C-type lamins had low levels of B-type lamins, and vice versa.
Permanent Link: http://hdl.handle.net/11104/0233409