Comparison of Protein Fractions in Seminal Plasma from Multiple Sperm Collections in Sterlet (Acipenser ruthenus)

0425839 - MBÚ 2014 RIV DE eng J - Journal Article
Shaliutina, A. - Hulák, M. - Li, P. - Šulc, Miroslav - Dzyuba, B. - Linhart, O.
Comparison of Protein Fractions in Seminal Plasma from Multiple Sperm Collections in Sterlet (Acipenser ruthenus).
Reproduction in Domestical Animals. Roč. 48, č. 1 (2013), s. 156-159. ISSN 0936-6768. E-ISSN 1439-0531
Institutional support: RVO:61388971
Keywords : TROUT ONCORHYNCHUS-MYKISS * SEMEN CHARACTERISTICS * SPERMATOZOA
Subject RIV: EE - Microbiology, Virology
Impact factor: 1.177, year: 2013

Contents Seminal plasma of sterlet Acipenser ruthenus was evaluated using comparative proteomics to characterize its protein fractions and to determine any influence of multiple sperm collections on these proteins. An experimental group of fish was used, in which sperm was collected three times at 5 h intervals. Protein fractions of seminal plasma were determined by SDS-gel electrophoresis (SDS-PAGE) and two-dimensional electrophoresis high-resolution gels (2D). At all stripping times, five protein bands with molecular weights of 93, 53, 48, 33 and 28 kDa were identified using SDS-PAGE. No significant differences (p > 0.05) in relative mass of protein bands among collections were observed. At the third collection, 20 protein spots were detected from the two-dimensional gels, compared to 17 found at the first and second collections. Ten protein spots, from the third stripping, were analysed
Permanent Link: http://hdl.handle.net/11104/0231673