Co-encapsulation of human serum albumin and superparamagnetic iron oxide in PLGA nanoparticles: Part I. Effect of process variables on the mean size

0425219 - ÚMCH 2014 RIV GB eng J - Journal Article
Shubhra, Q. T. H. - Kardos, A. F. - Feczkó, T. - Macková, Hana - Horák, Daniel - Tóth, J. - Dósa, G. - Gyenis, J.
Co-encapsulation of human serum albumin and superparamagnetic iron oxide in PLGA nanoparticles: Part I. Effect of process variables on the mean size.
Journal of Microencapsulation. Roč. 31, č. 2 (2014), s. 147-155. ISSN 0265-2048. E-ISSN 1464-5246
R&D Projects: GA AV ČR(CZ) KAN401220801
Institutional support: RVO:61389013
Keywords : albumin * encapsulation * PLGA (poly d,l-lactic-co-glycolic acid
Subject RIV: JB - Sensors, Measurment, Regulation
Impact factor: 1.585, year: 2014

PLGA (poly d,l-lactic-co-glycolic acid) nanoparticles (NPs) encapsulating magnetite nanoparticles (MNPs) along with a model drug human serum albumin (HSA) were prepared by double emulsion solvent evaporation method. This Part I will focus on size and size distribution of prepared NPs, whereas encapsulation efficiency will be discussed in Part II. It was found that mean hydrodynamic particle size was influenced by five important process variables. To explore their effects, a five-factorial, three-level experimental design and statistical analysis were carried out using STATISTICA® software. Effect of process variables on the mean size of nanoparticles was investigated and finally conditions to minimize size of NPs were proposed. GAMS™/MINOS software was used for optimization. The mean hydrodynamic size of nanoparticles ranged from 115 to 329 nm depending on the process conditions. Smallest possible mean particle size can be achieved by using low polymer concentration and high dispersion energy (enough sonication time) along with small aqueous/organic volume ratio.
Permanent Link: http://hdl.handle.net/11104/0231628