Gadolinium- and manganite-based contrast agents with fluorescent probes for both magnetic resonance and fluorescence imaging of pancreatic islets: a comparative study

0425115 - FZÚ 2014 RIV DE eng J - Journal Article
Berková, Z. - Jirák, D. - Zacharovová, K. - Lukeš, I. - Kotková, Z. - Kotek, J. - Kačenka, M. - Kaman, Ondřej - Řehoř, I. - Hájek, M. - Saudek, F.
Gadolinium- and manganite-based contrast agents with fluorescent probes for both magnetic resonance and fluorescence imaging of pancreatic islets: a comparative study.
ChemMedChem. Roč. 8, č. 4 (2013), s. 614-621. ISSN 1860-7179. E-ISSN 1860-7187
Institutional support: RVO:68378271
Keywords : contrast agents * gadolinium * magnetic resonance imaging * manganite * pancreatic islets
Subject RIV: BM - Solid Matter Physics ; Magnetism
Impact factor: 3.046, year: 2013

Three magnetic resonance (MR)/fluorescence imaging probes were tested for visualization, cellular distribution, and survival of labeled pancreatic islets invitro and following transplantation. As T1 contrast agents (CAs), Gd(III) complexes linked to -cyclodextrin (Gd-F-CD) or bound to titanium dioxide (TiO2@RhdGd) were tested. As a T2 CA, perovskite manganite nanoparticles (LSMO@siF@si) were examined. Fluorescein or rhodamine was incorporated as a fluorescent marker in all probes. Islets labeled with Gd(III) CAs were visible as hyperintense spots on MR invitro, but detection invivo was inconclusive. Islets labeled with LSMO@siF@si CA were clearly visible as hypointense spots or areas on MR scans invitro as well as invivo. All CAs were detected inside the islet cells by fluorescence. Although the vitality and function of the labeled islets was not impaired by any of the tested CAs, results indicate that LSMO@siF@si CA is a superior marker for islet labeling.
Permanent Link: http://hdl.handle.net/11104/0231053