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Multidimensional separation methods in analysis of complex biological mixtures

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    0424665 - ÚIACH 2014 CZ eng D - Thesis
    Duša, Filip
    Multidimensional separation methods in analysis of complex biological mixtures.
    Ústav analytické chemie AV ČR, v. v. i. Defended: Brno. 29.01.2014. - Brno: Masaryk university, Faculty of Science, 2014. 92 s.
    R&D Projects: GA MŠk(CZ) EE2.3.20.0182
    Institutional support: RVO:68081715
    Keywords : isoelectric focusing * liquid chromatography * two-dimensional separations * sample pretreatment * proteins
    Subject RIV: CB - Analytical Chemistry, Separation

    This work deals with two-dimensional separations of peptides and proteins from biological matrices. Theoretical introduction describes extraction of proteins from biological matrices, modes of isoelectric focusing (IEF) and liquid chromatography (LC) used for fractionation in two-dimensional separation schemes, and current papers dealing with combination of IEF and LC. In the results chapter there are four papers, which passed reviews and have been published in international scientific journals.
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    0424665.pdf49 MBPublisher’s postprintrequire
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