Novel human multiple myeloma cell line UHKT-893

0423264 - ÚMG 2014 RIV GB eng J - Journal Article
Uherková, L. - Vančurová, I. - Vyhlídalová, I. - Pleschnerová, M. - Špička, I. - Mihalová, R. - Březinová, J. - Hodný, Zdeněk - Čermáková, K. - Polanská, V. - Marinov, I. - Jedelský, P.L. - Kuželová, K. - Stöckbauer, P.
Novel human multiple myeloma cell line UHKT-893.
Leukemia Research. Roč. 37, č. 3 (2013), s. 320-326. ISSN 0145-2126. E-ISSN 1873-5835
Institutional support: RVO:68378050
Keywords : human myeloma cell line * human multiple myeloma * plasma cell * IL-6 dependence * immunoglobulin * free light chain
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 2.692, year: 2013

We established and characterized a new IL-6 dependent multiple myeloma (MM) cell line UHKT-893 from the bone marrow of a relapsed 57-year-old woman. Using nephelometry, cells with plasma cell phenotype and morphology were found to secrete IgG and free kappa (κ)-light chain of immunoglobulin. κ-Light chain was also recognized intracellularly by flow cytometry and by mass spectrometry. VH4-39 region of IgVH genes was rearranged and somatically hypermutated. Cytogenetic analysis of cells revealed new chromosome abnormalities in all breakpoints unique in both MM patients and cell lines – t(1;6), t(1;11), t(5;15), t(5;21), +der(11;15) and der(16). IL-6 independent subline UHKT-893a was established by adaptation to descending IL-6 concentration, while the original cell line keeps on maintaining its IL-6 dependency. The cell line provides a suitable material for cellular and molecular studies of tumor abnormalities, with potentially unique mutagenic features of myeloma disease. It may be utilized for human hybridoma construction and vaccine development. Both IL-6 dependent and independent cell clones represent an important model for studies of myeloma cell growth and resistance emerging during targeted therapy.
Permanent Link: http://hdl.handle.net/11104/0229382