Utilization of micropreparative fast focusing by a new wide pH range electrolyte system based on bidirectional isotachophoresis

0423163 - ÚIACH 2014 RIV CZ eng C - Conference Paper (international conference)
Vykydalová, Marie - Duša, Filip - Horká, Marie - Šlais, Karel
Utilization of micropreparative fast focusing by a new wide pH range electrolyte system based on bidirectional isotachophoresis.
Chemické Listy. Roč. 107, Issue s3.. Praha: Česká společnost chemická, 2013, s. 471-472. ISSN 0009-2770. E-ISSN 1213-7103.
[CECE Junior 2013. Brno (CZ), 12.11.2013-13.11.2013]
R&D Projects: GA MV VG20112015021
Institutional support: RVO:68081715
Keywords : bidirectional isotachophoresis * electrolyte system * preparative focusing
Subject RIV: CB - Analytical Chemistry, Separation

We suggest the possibility of practical utilization of a new electrolyte system for fast preparative focusing in wide pH range based on bidirectional isotachophoresis. The focusing occurs on nonwoven fabric strip positioned in an open horizontal V-shaped trough. It is based on bidirectional ITP with multiple counter ions and spacers created from commercially available simple buffers. Milk spiked with ampicillin was used as a sample and high performance liquid chromatography was as a second dimension for analysis of fast preparative focusing fractions. Speed, easy fraction handling, and possibility of pre-concentration of analytes from a raw sample are the benefits of this technique.
Permanent Link: http://hdl.handle.net/11104/0229245