High pressure modification of the simple automated liquid chromatographic system for splitless nano column gradient separations

0422993 - ÚIACH 2014 RIV CZ eng C - Conference Paper (international conference)
Šesták, Jozef - Kahle, Vladislav
High pressure modification of the simple automated liquid chromatographic system for splitless nano column gradient separations.
Chemické Listy. Roč. 107, Issue s3.. Praha: Česká společnost chemická, 2013, s. 438-440. ISSN 0009-2770. E-ISSN 1213-7103.
[CECE Junior 2013. Brno (CZ), 12.11.2013-13.11.2013]
R&D Projects: GA MV VG20112015021
Institutional support: RVO:68081715
Keywords : liquid chromatography * nano column gradient separations * gradient separation
Subject RIV: CB - Analytical Chemistry, Separation

Our simple liquid chromatographic system for splitless gradient nanocolumn separations at backpressure up to 50 bars was modified to run analysis under the pressure up to 300 bars. Programmable syringe pump was equipped with a 250-L high-pressure syringe and mobile phase gradients were created and stored in relatively long capillary. Available linear gradients of volumes from 5 to 50 L traced by uracil are presented. Sample introduction was performed as separate step and real large volume of sample (tens of microliters) could be injected and preconcentrated on-column. Relative standard deviation of retention times and peak areas were received (RSD < 0.3 % and RSD < 5 % respectively).
Permanent Link: http://hdl.handle.net/11104/0229100