IEF and HPLC-based methods for efficient bacterial characterization

0422799 - ÚIACH 2014 RIV CZ eng C - Conference Paper (international conference)
Kubesová, Anna - Šalplachta, Jiří - Moravcová, Dana - Horká, Marie
IEF and HPLC-based methods for efficient bacterial characterization.
Chemické Listy. Roč. 107, Issue s3.. Praha: Česká společnost chemická, 2013, s. 391-392. ISSN 0009-2770. E-ISSN 1213-7103.
[CECE Junior 2013. Brno (CZ), 12.11.2013-13.11.2013]
R&D Projects: GA MŠMT(CZ) EE2.3.20.0182; GA MV VG20102015023; GA MV VG20112015021
Institutional support: RVO:68081715
Keywords : Pectobacterium * Dickeya species * gel isoelectric focusing * liquid chromatography
Subject RIV: CB - Analytical Chemistry, Separation

Pectobacterium and Dickeya species are responsible for blackleg and tuber soft rot diseases on crop and ornamental plants. Recently, a more virulent and aggressive Dickeya type of E. chrysanthemi was described and it becomes serious problem for potato production in Europe. In this study, we suggest procedures using two commonly available laboratory techniques, gel isoelectric focusing and liquid chromatography, for differentiation and characterization of Pectobacterium and Dickeya species. We have confirmed that fingerprinting approach can be used for bacterial differentiation, which can be further used in rapid diagnosis of plant disease.
Permanent Link: http://hdl.handle.net/11104/0228934