Formation of ROS and RNS in Water Electro-Sprayed through Transient Spark Discharge in Air and their Bactericidal Effects

0421972 - ÚFP 2014 RIV DE eng J - Journal Article
Machala, Z. - Tarabová, B. - Hensel, K. - Doležalová, Eva - Šikurová, L. - Lukeš, Petr
Formation of ROS and RNS in Water Electro-Sprayed through Transient Spark Discharge in Air and their Bactericidal Effects.
Plasma Processes and Polymers. Roč. 10, č. 7 (2013), s. 649-659. ISSN 1612-8850. E-ISSN 1612-8869
R&D Projects: GA AV ČR IAAX00430802; GA ČR(CZ) GD104/09/H080; GA MŠMT(CZ) MEB0810116
Institutional support: RVO:61389021
Keywords : Plasma electrospray * water * bacteria * hydrogen peroxide * peroxynitrite * cold plasma * water electro-spray
Subject RIV: BL - Plasma and Gas Discharge Physics
Impact factor: 2.964, year: 2013
http://dx.doi.org/10.1002/ppap.201200113

Chemical and bactericidal effects induced by plasma in water upon electro-spraying through DC-driven positive transient spark discharge in air were investigated. Inactivation of E. coli was determined in dependence on pH (controlled by buffers) and correlated with chemical changes induced in water. Productions of hydrogen peroxide, nitrites, nitrates, peroxynitrites and pH changes were determined, and the extent of oxidative stress induced in bacteria was evaluated. The degree of inactivation and oxidative damage of bacteria increased with the increasing acidity of the solution. Acidified nitrites interacting with hydrogen peroxide were determined as the most important bactericidal ROS/RNS agents in plasma-treated water. A possible role of peroxynitrites, ozone and metal nanoparticles is discussed.
Permanent Link: http://hdl.handle.net/11104/0228215