Plasma Membrane Density of GABA(B)-R1a, GABA(B)-R1b, GABA-R2 and Trimeric G-proteins in the Course of Postnatal Development of Rat Brain Cortex

0420970 - FGÚ 2014 RIV CZ eng J - Journal Article
Dlouhá, Kateřina - Kagan, Dmytro - Roubalová, Lenka - Ujčíková, Hana - Svoboda, Petr
Plasma Membrane Density of GABA(B)-R1a, GABA(B)-R1b, GABA-R2 and Trimeric G-proteins in the Course of Postnatal Development of Rat Brain Cortex.
Physiological Research. Roč. 62, č. 5 (2013), s. 547-559. ISSN 0862-8408. E-ISSN 1802-9973
R&D Projects: GA ČR(CZ) GAP207/12/0919; GA ČR(CZ) GBP304/12/G069
Institutional support: RVO:67985823
Keywords : GABAB-receptors * postnatal development * rat brain cortex * G-proteins * Na, K-ATPase
Subject RIV: CE - Biochemistry
Impact factor: 1.487, year: 2013

GABAB-R1a, GABAB-R1b, GABAB-R2, Gi1/Gi2alpha, Gi3alpha, Goalpha, Gzalpha and Gbeta subunit protein content was determined by quantitative immunoblotting in PM isolated from brain cortex of rats of different ages: between postnatal day1 (PD1) and 90 (PD90). PM density of GABAB-R1alpha, GABAB-R2, Gi1/Gi2alpha, Gi3alpha, Goalpha, Gzalpha and Gbeta was high at birth. The intensity of GABAB-R1a and GABAB-R2 subunits signal decreased between the birth and PD15: to 55 % (R1a, **) and 51 % (R2, **), respectively. Contrarily, PM level of the cognate G-proteins Gi1/Gi2alpha, Gi3alpha, Goalpha, Gzalpha and Gbeta was unchanged in the course of the whole postnatal period of brain cortex development. Maturation of GABAB-R cascade was substantially different from ontogenetic profile of Na, K-ATPase, which was low at birth and increased between the birth and PD25. In adult rats, membrane content of Na, K-ATPase was 3-times higher than around the birth
Permanent Link: http://hdl.handle.net/11104/0227490