Quantification of active caspases in stem cells: single cell analysis at femtogram level

0399403 - ÚIACH 2014 DE eng A - Abstract
Adamová, Eva - Lišková, Marcela - Klepárník, Karel - Hampl, Aleš - Matalová, Eva
Quantification of active caspases in stem cells: single cell analysis at femtogram level.
The Abstracts of the World Conference on Regenerative Medicine 2013. Leipzig: Fraunhofer Institute for Cell Therapy and Immunology, 2013. s. 358.
[World Conference on Regenerative Medicine 2013 /WCRM 2013/. 23.10.2013-25.10.2013, Leipzig]
R&D Projects: GA ČR GAP206/11/2377; GA ČR GAP304/11/1418
Institutional support: RVO:68081715 ; RVO:67985904 ; RVO:68378041
Keywords : caspases * cell analysis * cancer cell
Subject RIV: CB - Analytical Chemistry, Separation; EA - Cell Biology (UZFG-Y); EB - Genetics ; Molecular Biology (UEM-P)

There are several methods for caspase detection in tissue sections and individual cells. However, quantification lags behind qualitative evaluation of active caspases. Therefore, first we aimed to develop/modify a precise technique for active caspase measurement, and second to apply the procedure to express amount of active caspase per cell in cell populations. Further, we improved the method for single cell analysis. As one of model systems, stem cells (neural crest derived) were selected and challenged by camptothecin, a cytotoxic alkaloid used as natural anticancer drug.
Permanent Link: http://hdl.handle.net/11104/0226704