Cytotoxic Constituents of Pachyrhizus tuberosus from Peruvian Amazon

0399185 - ÚOCHB 2014 RIV US eng J - Journal Article
Leuner, O. - Havlík, J. - Buděšínský, Miloš - Vrkoslav, Vladimír - Chu, J. - Bradshaw, T. D. - Hummelová, J. - Mikšátková, P. - Lapčík, O. - Valterová, Irena - Kokoška, L.
Cytotoxic Constituents of Pachyrhizus tuberosus from Peruvian Amazon.
Natural Product Communications. Roč. 8, č. 10 (2013), s. 1423-1426. ISSN 1934-578X. E-ISSN 1555-9475
Grant - others:GA ČR(CZ) GA525/09/0994
Program: GA
Institutional support: RVO:61388963
Keywords : Pachyrrhizus sp. * Amazonian yam bean * isoflavonoid * rotenoid
Subject RIV: CC - Organic Chemistry
Impact factor: 0.924, year: 2013

Investigations into the chemical constituents of the seeds of the neglected tuber crop Pachyrhizus tuberosus (Leguminosae) resulted in the isolation of seven components: five rotenoids [12a-hydroxyerosone (1), 12a-hydroxydolineone (2), erosone (3), 12a-hydroxyrotenone (4) and rotenone (6)], a phenylfuranocoumarin [pachyrrhizine (5)] and an isoflavanone [neotenone (7)]. The compounds were isolated using several chromatography techniques and characterized and verified by NMR and HPLC/MS. The MTT assay was used to examine the selective cytotoxic effects of the methanolic P. tuberosus extract and isolated compounds in two human cancer cell lines [breast (MCF-7) and colorectal (HCT-116)] and in non-transformed human fibroblasts (MRC-5); IC50 values were calculated. The methanolic P. tuberosus extract displayed respectable cytotoxic effects against HCT-116 and MCF-7 cells with IC50 values of 7.3 and 6.3 mu g/mL, respectively. Of the compounds, 6 exacted greatest cytotoxicity and selectivity towards the cancer cell lines tested, yielding IC50 values of 0.3 mu g/mL against both MCF-7 and HCT-116 cells, and a 6-fold reduced activity against MRC-5 fibroblasts. Compound 4 also demonstrated cytotoxicity against MCF-7 and HCT-116 (1.1 and 1.8 mu g/mL, respectively), and reduced cytotoxicity towards MRC-5 cells (7.5 mu g/mL). The results revealed from the in vitro cytotoxic MTT assay are worthy of further antitumor investigation.
Permanent Link: http://hdl.handle.net/11104/0226524