The use of new surface-modified poly(2-hydroxyethyl methacrylate) hydrogels in tissue engineering: Treatment of the surface with fibronectin subunits versus Ac-CGGASIKVAVS-OH, cysteine, and 2-mercaptoethanol modification

0397918 - ÚEM 2015 RIV US eng J - Journal Article
Kubinová, Šárka - Horák, Daniel - Vaněček, Václav - Plichta, Zdeněk - Proks, Vladimír - Syková, Eva
The use of new surface-modified poly(2-hydroxyethyl methacrylate) hydrogels in tissue engineering: Treatment of the surface with fibronectin subunits versus Ac-CGGASIKVAVS-OH, cysteine, and 2-mercaptoethanol modification.
Journal of Biomedical Materials Research. Part A. Roč. 102, č. 7 (2014), s. 2315-2323. ISSN 1549-3296. E-ISSN 1552-4965
R&D Projects: GA ČR GAP304/11/0731; GA ČR GAP304/11/0653; GA ČR GA13-00939S
Institutional support: RVO:68378041 ; RVO:61389013
Keywords : 2-hydroxyethyl methacrylate * IKVAV (Ile-Lys-Val-Ala-Val) peptide * fibronectin
Subject RIV: FH - Neurology; CD - Macromolecular Chemistry (UMCH-V)
Impact factor: 3.369, year: 2014

The objective of this study was to investigate the cell-adhesive properties of biomimetic ligands, such as laminin-derived Ac-CGGASIKVAVS-OH (SIKVAV) peptide and fibronectin subunits (Fn), as well as small molecules exemplified by 2-mercaptoethanol (ME) and cysteine (Cys), immobilized on a copolymer of 2-hydroxyethyl methacrylate (HEMA) with 2-aminoethyl methacrylate (AEMA) by a maleimide-thiol coupling reaction. The results show that the immobilization of SIKVAV and Fn-subunits onto superporous P(HEMA-AEMA) hydrogels via a GMBS coupling reaction improves cell adhesive properties. The high proliferative activity observed on Fn-modified hydrogels suggests that the immobilized Fn-subunits maintain their bioactivity and thus represent a promising tool for application in tissue engineering
Permanent Link: http://hdl.handle.net/11104/0233918