Mechanistic basis of infertility of mouse intersubspecific hybrids

0397570 - ÚMG 2014 RIV US eng J - Journal Article
Bhattacharyya, Tanmoy - Gregorová, Soňa - Mihola, Ondřej - Anger, Martin - Šebestová, J. - Denny, P. - Šimeček, Petr - Forejt, Jiří
Mechanistic basis of infertility of mouse intersubspecific hybrids.
Proceedings of the National Academy of Sciences of the United States of America. Roč. 110, č. 6 (2013), E468-E477. ISSN 0027-8424. E-ISSN 1091-6490
R&D Projects: GA MŠMT(CZ) LD11079; GA MŠMT(CZ) 1M0520; GA MŠMT ED1.1.00/02.0109; GA ČR(CZ) GPP305/11/P630; GA ČR GA523/09/0743
Grant - others:AV ČR(CZ) Premium Academiae of the Academy of Sciences of the Czech Republic; GA MŠk(CZ) ED1.1.00/02.0068
Program: Akademická prémie - Praemium Academiae; ED
Institutional research plan: CEZ:AV0Z50520514
Institutional support: RVO:68378050 ; RVO:67985904
Keywords : meiosis * meiotic sex chromosome inactivation * Prdm9 * chromosome substitution strains * Haldane's rule
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 9.809, year: 2013
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3568299/

According to the Dobzhansky-Muller model, hybrid sterility is a consequence of the independent evolution of related taxa resulting in incompatible genomic interactions of their hybrids. The model implies that the incompatibilities evolve randomly, unless a particular gene or nongenic sequence diverges much faster than the rest of the genome. Here we propose that asynapsis of heterospecific chromosomes in meiotic prophase provides a recurrently evolving trigger for the meiotic arrest of interspecific F1 hybrids. We observed extensive asynapsis of chromosomes and disturbance of the sex body in >95% of pachynemas of Mus m. musculus x Mus m. domesticus sterile F1 males. Asynapsis was not preceded by a failure of double-strand break induction, and the rate of meiotic crossing over was not affected in synapsed chromosomes. DNA double-strand break repair was delayed or failed in unsynapsed autosomes, and misexpression of chromosome X and chromosome Y genes was detected in single pachynemas and by genome-wide expression profiling. Oocytes of F1 hybrid females showed the same kind of synaptic problems but with the incidence reduced to half. Most of the oocytes with pachytene asynapsis were eliminated before birth. We propose the heterospecific pairing of homologous chromosomes as a preexisting condition of asynapsis in interspecific hybrids. The asynapsis may represent a universal mechanistic basis of F1 hybrid sterility manifested by pachytene arrest. It is tempting to speculate that a fast-evolving subset of the noncoding genomic sequence important for chromosome pairing and synapsis may be the culprit.
Permanent Link: http://hdl.handle.net/11104/0227589