Evaluation of tumor suppressor gene expressions and aberrant methylation in the colon of cancer-induced rats: a pilot study

0397116 - ÚEM 2014 RIV NL eng J - Journal Article
Vymetálková, Veronika - Vannucci, Luca - Korenková, Vlasta - Procházka, Pavel - Slyšková, Jana - Vodičková, Ludmila - Rusňáková, Vendula - Bielik, Ludovít - Burocziová, Monika - Rossmann, Pavel - Vodička, Pavel
Evaluation of tumor suppressor gene expressions and aberrant methylation in the colon of cancer-induced rats: a pilot study.
Molecular Biology Reports. Roč. 40, č. 10 (2013), s. 5921-5929. ISSN 0301-4851. E-ISSN 1573-4978
R&D Projects: GA AV ČR IAA500200917; GA ČR GPP304/11/P715
Institutional research plan: CEZ:AV0Z50390703; CEZ:AV0Z50520701; CEZ:AV0Z50390512
Institutional support: RVO:61388971 ; RVO:68378041 ; RVO:86652036
Keywords : colorectal cancer * rats * mRNA expression
Subject RIV: EB - Genetics ; Molecular Biology; EB - Genetics ; Molecular Biology (BU-J); EB - Genetics ; Molecular Biology (MBU-M)
Impact factor: 1.958, year: 2013

The aim of this pilot study was to analyze expression profile and promoter methylation status of important tumor suppressor and DNA repair genes in the early stages of experimentally induced colorectal carcinogenesis. Rats were treated with azoxymethane (AOM), dextran sodium sulphate (DSS) or with their combination, and sacrificed 1 or 4 months post-treatment period. Present data suggest that down-regulation of Apc and MMR genes are prerequisite for the development of CRC. In this study we addressed for the first time early functional alterations of tumor suppressor genes with underlying epigenetic mechanisms in experimentally induced CRC in rats.
Permanent Link: http://hdl.handle.net/11104/0225351