Exploring chip-capillary electrophoresis-laser-induced fluorescence field-deployable platform flexibility: Separations of fluorescent dyes by chip-based non-aqueous capillary electrophoresis

0395838 - ÚIACH 2014 RIV NL eng J - Journal Article
Nuchtavorn, N. - Smejkal, Petr - Breadmore, M. C. - Guijt, R. M. - Doble, P. - Bek, F. - Foret, František - Suntornsuk, L. - Macka, M.
Exploring chip-capillary electrophoresis-laser-induced fluorescence field-deployable platform flexibility: Separations of fluorescent dyes by chip-based non-aqueous capillary electrophoresis.
Journal of Chromatography A. Roč. 1286, APR (2013), s. 216-221. ISSN 0021-9673. E-ISSN 1873-3778
R&D Projects: GA ČR(CZ) GBP206/12/G014
Institutional support: RVO:68081715
Keywords : microfluidic chip CE * capillary electrophoresis * NACE * LIF detection
Subject RIV: CB - Analytical Chemistry, Separation
Impact factor: 4.258, year: 2013

We studied the chip compatibility with organic solvents (i.e. MeOH, ACN, DMF and DMSO) and demonstrated the chip compatibility with DMSO as a non-volatile and non-hazardous solvent with satisfactory stability of migration times over 50 h. The generic CE capability is illustrated with separations of fluorescent basic blue dyes methylene blue (MB), toluidine blue (TB), nile blue (NB) and brilliant cresyl blue (BC). Further, the effects of the composition of the background electrolyte (BGE) on the separation were studied, including the contents of water (0-30%) and buffer composition. In background electrolytes containing typically 80 mmol/L ammonium acetate and 870 mmol/L acetic acid in 100% DMSO baseline separation of the dyes were achieved in 40 s. Linearity was documented in the range of 5-28 mu mol/L, 10-100 mu mol/L, 1.56-50 nmol/L and 5-75 nmol/L (r(2) values in the range 0.974-0.999), and limit of detection (LOD) values were 90 nmol/L, 1 mu mol/L 1.4 nmol/L, and 2 nmol/L for MB, TB, NB and BC, respectively. (c) 2013 Elsevier B.V. All rights reserved.
Permanent Link: http://hdl.handle.net/11104/0223768