Capillary electrophoresis in the N-glycosylation analysis of biopharmaceuticals

0395807 - ÚIACH 2014 RIV GB eng J - Journal Article
Guttman, András
Capillary electrophoresis in the N-glycosylation analysis of biopharmaceuticals.
TrAC-Trends in Analytical Chemistry. Roč. 48, JUL-AUG (2013), s. 132-143. ISSN 0165-9936. E-ISSN 1879-3142
Institutional support: RVO:68081715
Keywords : automated workflow * biopharmaceuticals * capillary electrophoresis
Subject RIV: CB - Analytical Chemistry, Separation
Impact factor: 6.612, year: 2013

In N-glycosylation analysis of biopharmaceuticals, analytical need depends on the phase of the manufacturing process. All important glycoanalysis steps are thoroughly discussed. Carbohydrate sequencing by exoglycosidase arrays is described in conjunction with capillary electrophoresis (CE) to identify linkage and positional isomers. A possible automated workflow for N-glycosylation based on CE is outlined. (c) 2013 Elsevier Ltd. All rights reserved.
Permanent Link: http://hdl.handle.net/11104/0223762