Nucleotide Excision Repair Is Not Induced in Human Embryonic Lung Fibroblasts Treated with Environmental Pollutants

0395392 - ÚEM 2014 RIV US eng J - Journal Article
Rössner ml., Pavel - Mrhálková, Andrea - Uhlířová, Kateřina - Špátová, Milada - Rössnerová, Andrea - Líbalová, Helena - Schmuczerová, Jana - Milcová, Alena - Topinka, Jan - Šrám, Radim
Nucleotide Excision Repair Is Not Induced in Human Embryonic Lung Fibroblasts Treated with Environmental Pollutants.
PLoS ONE. Roč. 8, č. 7 (2013), e69197. ISSN 1932-6203. E-ISSN 1932-6203
R&D Projects: GA ČR GAP503/11/0084
Institutional support: RVO:68378041
Keywords : environmental pollutants * DNA * expression of DNA
Subject RIV: DN - Health Impact of the Environment Quality
Impact factor: 3.534, year: 2013

We tested response of normal human embryonic lung fibroblasts (HEL12469 cells) to treatment with benzo[a]pyrene (B[a]P) and extractable organic matter (EOM) from ambient air particles (PM2.5) collected in two Czech cities differing in levels and sources of air pollution. We analyzed multiple endpoints associated with exposure to PAHs: bulky DNA adducts and the nucleotide excision repair (NER) response [expression of XPE, XPC and XPA genes on the level of mRNA and proteins, unscheduled DNA synthesis (UDS)]. EOMs were collected in the winter and summer of 2011 in two Czech cities with different levels and sources of air pollution. The levels of bulky DNA adducts were highest after treatment with B[a]P, followed by winter EOMs; their induction by summer EOMs was weak. The induction of both mRNA and protein expression was observed, with the most pronounced effects after treatment with B[a]P (–S9); the response induced by EOMs from both cities and seasons was substantially weaker. The expression of DNA repair genes was not accompanied by the induction of UDS activity. Our results indicate that the tested compounds induced low levels of DNA damage and affected the expression of NER genes; however, nucleotide excision repair was not induced.
Permanent Link: http://hdl.handle.net/11104/0224656