Bilateral elevation of interleukin-6 protein and mRNA in both lumbar and cervical dorsal root ganglia following unilateral chronic compression injury of the sciatic nerve

0394844 - BFÚ 2014 RIV GB eng J - Journal Article
Dubový, P. - Brázda, Václav - Klusáková, I. - Hradilová-Svíženská, I.
Bilateral elevation of interleukin-6 protein and mRNA in both lumbar and cervical dorsal root ganglia following unilateral chronic compression injury of the sciatic nerve.
Journal of Neuroinflammation. Roč. 10, č. 55 (2013). E-ISSN 1742-2094
Grant - others:GA MŠk(CZ) ED1.1.00/02.0068
Program: ED
Institutional research plan: CEZ:AV0Z50040702
Institutional support: RVO:68081707
Keywords : PRO-INFLAMMATORY CYTOKINES * SATELLITE GLIAL-CELLS * PAIN SENSATION LIKE
Subject RIV: BO - Biophysics
Impact factor: 4.902, year: 2013

Methods: We used unilateral chronic constriction injury (CCI) of rat sciatic nerve as a model for neuropathic pain. Immunofluorescence, ELISA, western blotting and in situ hybridization were used to investigate bilateral changes in IL-6 protein and mRNA in both lumbar (L4-L5) and cervical (C7-C8) dorsal root ganglia (DRG) following CCI. The operated (CCI) and sham-operated (sham) rats were assessed after 1, 3, 7, and 14 days. Withdrawal thresholds for mechanical hyperalgesia and latencies for thermal hyperalgesia were measured in both ipsilateral and contralateral hind and fore paws. Results: The ipsilateral hind paws of all CCI rats displayed a decreased threshold of mechanical hyperalgesia and withdrawal latency of thermal hyperalgesia, while the contralateral hind and fore paws of both sides exhibited no significant changes in mechanical or thermal sensitivity.
Permanent Link: http://hdl.handle.net/11104/0223016