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Separation of antibiotics present in biological fluids by capillary isoelectric focusing and high-performance liquid chromatography with UV detection

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    0393281 - ÚIACH 2014 eng A - Abstract
    Vykydalová, Marie - Horká, Marie - Růžička, F. - Duša, Filip - Moravcová, Dana - Kahle, Vladislav - Šlais, Karel
    Separation of antibiotics present in biological fluids by capillary isoelectric focusing and high-performance liquid chromatography with UV detection.
    HPLC 2013 Amsterdam. Book of Abstracts. 2013. s. 257-257.
    [International Symposium on High Performance Liquid Phase Separations and Related Techniques /39./. 16.06.2013-20.06.2013, Amsterdam]
    R&D Projects: GA MV VG20112015021
    Institutional support: RVO:68081715
    Keywords : antibiotics * HPLC * CIEF
    Subject RIV: CB - Analytical Chemistry, Separation

    We have detected antibiotics present in bovine blood and Mueller-Hinton broth according to their isoelectric points by CIEF. Then, we have determined vancomycin and ampicillin in milk and cream by CIEF and HPLC. We have to use solution-phase isoelectric focusing in non-woven fabric strip for pre-concentration of real samples before analysis.
    Permanent Link: http://hdl.handle.net/11104/0222000

     
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