Isoelectric focusing in continuously tapered fused silica capillary prepared by etching with supercritical water

0392235 - ÚIACH 2014 RIV US eng J - Journal Article
Šlais, Karel - Horká, Marie - Karásek, Pavel - Planeta, Josef - Roth, Michal
Isoelectric focusing in continuously tapered fused silica capillary prepared by etching with supercritical water.
Analytical Chemistry. Roč. 85, č. 9 (2013), s. 4296-4300. ISSN 0003-2700. E-ISSN 1520-6882
R&D Projects: GA ČR(CZ) GAP106/12/0522; GA MV VG20102015023
Institutional support: RVO:68081715
Keywords : capillary isoelectric focusing * resolution of ampholytes * supercritical water
Subject RIV: CB - Analytical Chemistry, Separation
Impact factor: 5.825, year: 2013

In capillary isoelectric focusing carried out in tapered capillaries, several times higher resolution of ampholytes can be obtained as compared with the resolution of the respective pair of ampholytes in conventional cylindrical capillaries. The tapered fused-silica capillaries were reproducibly prepared by etching with supercritical water, and they open the way to increase the number of separable compounds without resorting to excessively high voltage.
Permanent Link: http://hdl.handle.net/11104/0221156