Optimized method for identification of the proteomes secreted by cardiac cells

0392231 - ÚIACH 2014 RIV US eng J - Journal Article
Šťastná, Miroslava - Van Eyk, J.E.
Optimized method for identification of the proteomes secreted by cardiac cells.
Methods in molecular biology. Roč. 1005, č. 1005 (2013), s. 225-235. ISSN 1940-6029
Institutional support: RVO:68081715
Keywords : cardiac cells * secreted proteins * proteomic technology
Subject RIV: CB - Analytical Chemistry, Separation

There are several crucial steps that have to be followed to ensure successful secreted proteome detection and identification. Simultaneously with the optimization of the experimental conditions for various cell type culturing and subsequent cell conditioning to obtain conditioned medium with secreted proteins in vitro, the analytical separation methods for fractionation of complex protein mixture and mass spectrometry for protein identification are of high importance. Although optimized for neonatal cardiac myocytes, the general steps described in the following chapter can be adapted to other cell types as well.
Permanent Link: http://hdl.handle.net/11104/0221151