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Species-specific PCR primers for detection of Xanthomonas vesicatoria
- 1.0392154 - BC 2014 RIV NL eng J - Journal Article
Beran, P. - Mráz, Ivan
Species-specific PCR primers for detection of Xanthomonas vesicatoria.
Crop Protection. Roč. 43, Jan (2013), s. 213-215. ISSN 0261-2194. E-ISSN 1873-6904
R&D Projects: GA MZe QH71229
Institutional support: RVO:60077344
Keywords : Bacterial spot * Tomato * Pepper
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 1.539, year: 2013
This study presents the development of a PCR method for specific detection of Xanthomonas vesicatoria, causal agent of bacterial spot of tomato and pepper. Primer pair XV1F and XV1R was designed based on partial DNA sequence of the catalytic subunit of the ATP synthase gene atpD and tested using a collection of various bacterial pathogens mainly for tomato and pepper. After optimization of the PCR conditions, the 365 bp long fragment was only produced in X. vesicatoria samples. Therefore, the primers and protocol allow rapid and species-specific detection of X. vesicatoria.
Permanent Link: http://hdl.handle.net/11104/0221102
Number of the records: 1